By W.S. Hoar and D.J. Randall (Eds.)

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Hippocampus, Da Lage, 1958), and pars intermedia cells are sometimes found in the pars distalis (Olivereau and Ball, 1964). Two cell types may usually be distinguished by their staining reactions and often also by differences in shape and position. Staining characteristics vary with species; pars intermedia cells have been described as basophilic, staining for example with Aniline blue, or acidophilic (with 1. THE 51 PITUITARY GLAND azocarmine or orange G ) , or amphiphilic, taking both the blue and orange components in trichrome and tetrachrome techniques.

17 and note that the gonadotrops are ve granulation. The growth hornow greatly enlarged and filled with a dense AF mone cells seem less numerous. AF, X430. For details, see Olivereau (1967a). + (resembling those seen in mammals, Purves, 1966) in teleost gonadotrops following surgical gonadectomy ( Sokol, 1955; Schreibman, 1964) or administration of gonadal steroids (Geske, 1956). The Gonudotrops of Poecilia ktipinna. Like many cyprinodonts, the molly is viviparous, with approximately monthly cycles of gestation, parturition, and oocyte growth, and the young fish quickly reach sexual maturity, in a matter of a few months in males reared in good conditions.

THE PITUITARY GLAND 37 identified by their responses to thiourea (Section 11, B, 5 ) . On the ventral surface of this region, one finds many small chromophobic cells, with inactive nuclei, presumably undifferentiated cells. In the sexually mature fish, differentiated basophils are always found in this region (Fig. 2 ) , even during periods of sexual quiescence, when these cells assume an inactive appearance but are nevertheless recognizable as differentiated basophils. Thus, these ventral basophils would appear to be gonadotrops.

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