By Nicholas H. Acheson
This new, totally revised moment variation of Fundamentals of Molecular Virology is designed for college scholars studying approximately virology on the undergraduate or graduate point. Chapters disguise lots of the significant virus households, emphasizing the original good points of every virus family members. those chapters are designed to inform tales concerning the viruses lined, and contain details on discovery, ailments and pathogenesis, virus constitution, steps in viral replication, and interplay with mobile signaling pathways. This technique portrays the “personality” of every virus, aiding scholars to profit the fabric and to accumulate their wisdom of virology, beginning with smaller and less complicated viruses and continuing to extra advanced viruses.
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Additional info for Fundamentals of Molecular Virology
Virus genomes are either RNA or DNA, but not both There are many different viruses in the world, and probably all organisms on Earth can be infected by at least one virus. Viruses have a variety of distinct morphologies, genome and particle sizes, and mechanisms of replication. The smallest known viruses are 20 nanometers1 (nm) in diameter; their genomes contain fewer than 2000 nucleotides, and they code for as few as 2 proteins. 2 million nucleotides, and they code for over 1200 proteins. An overview of the variety of known viruses is given in Chapter 3.
Therefore, hemagglutination assays are much less sensitive than plaque assays, but they are rapid and cheap. They can also be used to detect antibodies that bind to viral surface antigens, because addition of such antibodies will inhibit hemagglutination. Virus particles can be seen and counted by electron microscopy A variety of staining or shadowing methods can be used to detect virus particles by electron microscopy. 4 Hemagglutination assay. Red blood cells (RBC; small orange circles in central tube) are mixed with virus (small green spheres), or with buffer, and are allowed to settle.
7. indd 15 one of the RNA strands in the double-stranded genome. It is theoretically possible that the doublestranded genome RNA could be denatured within the cell, allowing the positive-strand RNA to be translated; however, this is energetically unlikely and does not occur. These viruses therefore also package a virus-coded RNA-dependent RNA polymerase in the virion and bring this enzyme into the host cell along with the genome. This RNA polymerase can speciﬁcally recognize the doublestranded RNA genome and transcribe its negativestrand into positive-strand messenger RNAs.