By Moselio Schaechter

The table Encyclopedia of Microbiology goals to supply a reasonable and prepared entry to a wide number of microbiological subject matters inside one set of covers. this useful desk-top reference brings jointly an exceptional choice of paintings via the head scientists within the box. protecting themes starting from the elemental technology of microbiology to the present ''hot'' themes within the box. * offers a vast, simply available point of view on quite a lot of microbiological subject matters * A synthesis of the broadest issues from the great and multi-volumed Encyclopedia of Microbiology, moment version * worthwhile source in getting ready for lectures, writing reviews, or drafting supply purposes

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The T-complex contains information for (i) export across the A. tumefaciens cell envelope via a dedicated transport system, (ii) movement through the plant plasma membrane and cytosol, (iii) delivery to the plant nuclear pore, and (iv) integration into the plant genome. Once integrated into the plant genome, T-DNA genes are expressed and the resulting gene products ultimately disrupt the balance of two endogenous plant hormones that synergistically coordinate plant cell growth and division events.

37–51. Academic Press, San Diego, CA. Christie, P. J. (1997). The Agrobacterium tumefaciens T-complex transport apparatus: A paradigm for a new family of multifunctional transporters in eubacteria. J. Bacteriol. 179, 3085–3094. Christie, P. , and Covacci, A. (1998). Bacterial type IV secretion systems: Systems utilizing components of DNA conjugation machines for export of virulence factors. In “Cellular Microbiology” (P. Cossart, P. Boquet, S. Normark, and R. ). ASM Press, Washington, DC. Christie, P.

This underscores the notion that interkingdom DNA transfer is a complex process dependent on a genetic interplay between A. tumefaciens and host cells. Fortunately, many of the agonomically important species are readily transformable, but additional efforts are needed to overcome the current obstacles impeding efficient transformation of other species of interest. B. Other applications Agrobacterium tumefaciens is increasingly used to characterize and isolate novel plant genes by an approach termed T-DNA tagging.

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