By Frank H. Stephenson

Essential for all Biotechnology scholars and technicians. It walks readers in the course of the minefield of laboratory calculations, aiding to avoid the kinds of mistakes that can suggest the lack of weeks of labor. Key Features:* issues variety from simple clinical notations to advanced topics like nucleic acid chemistry and recombinant DNA expertise* every one bankruptcy incorporates a short clarification of the concept that and covers priceless definitions, thought and motive for every kind of calculation* contemporary functions of the approaches and computations in medical, educational, commercial and simple study laboratories are pointed out during the textNew to this Edition:* up to date and elevated insurance of actual time PCR and the maths used to degree gene expression* extra pattern difficulties in each bankruptcy for readers to perform ideas

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Extra info for Calculations for Molecular Biology and Biotechnology, Second Edition: A Guide to Mathematics in the Laboratory 2e

Sample text

Coli. 75 mL sample is withdrawn and its OD at 550 nm (OD550) is determined. (Beyond a certain OD550 reading, an increase in cell density no longer results in a linear increase in absorbance. 5. 9 mL of tryptone broth. 8 mL of tryptone broth. 1 mL is withdrawn and spread onto an agar plate. Following overnight incubation of that plate, 420 colonies are counted. 1 In the example just given, what is the dilution of cells in the second tube? 1 The dilutions can be represented as fractions. 0 mL). Multiple dilutions are multiplied together to give the dilution in the final sample.

04 g of NaOH is dissolved in a final volume of 50 mL of distilled water. 15 M solution? 15 moles of NaCl per liter. Since we want to know how many moles are in 50 mL, we need to use a conversion factor to convert liters to milliliters. 15 mol  1000 mL L Notice that terms on the right side of the equation cancel except for moles. 0075 moles of NaCl. 1 Sodium citrate and sodium phosphate dibasic can come in hydrated forms. ’ That is, the compounds have water molecules attached to them. 1). You will find the compound’s molecular weight listed on the container’s label as the FW.

04 1000 Multiply both sides of the equation by 50. This cancels the 50 in the denominator on the right side of the equation. Simplify the equation. 04 g of NaOH is dissolved in a final volume of 50 mL of distilled water. 15 M solution? 15 moles of NaCl per liter. Since we want to know how many moles are in 50 mL, we need to use a conversion factor to convert liters to milliliters. 15 mol  1000 mL L Notice that terms on the right side of the equation cancel except for moles. 0075 moles of NaCl.

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