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If the object height is large enough (for example bigger than several micrometers) the artifact due to the pyramidal shape of the tip can appear as shadows (Hyde, Jacobs & Compton, 2004). , 2003). As another example, Fig. 4 shows the topography of organosilane layer photochemically grafted to O-terminated monocrystalline diamond. This image contains shadows near each cluster and the globular particles do not look like a sphere but rather like facetted crystals. We have seen that feature width is inherently overestimated in AFM.

M. D. (2006). Microstructurehardened silver nanowires. NANOLETTERS, Vol. 6, No. 3, pp. 468-472. ; Feijen, J. (2008). Mechanical properties of native and cross-linked type I collagen fibrils. BIOPHYSICAL JOURNAL, Vol. 94, No. 6, pp. ; Cao, Y. & Li H. (2011). Title: Facile Method of Constructing Polyproteins for Single-Molecule Force Spectroscopy Studies. LANGMUIR, Vol. 27, No. 10, pp. 57135718. ; Metselaar, G. ; et al. (2005). SFM characterization of poly(isocyanodipeptide) single polymer chains in controlled environments: Effect of tip adhesion and chain swelling.

2 Viscoelasticity and adhesion forces measurements Contact mode force spectroscopy is based on the detection of the static or quasi-static cantilever bending. Here, “quasi-static” has to be read in terms of relatively slow oscillations, well below the cantilever resonance. In such cases, informations are extracted directly and only from the cantilever deflection that “measures” the applied force. Dynamic mode AFM allows one to go further into the tip-sample interaction details and, in addition, the oscillation amplitude and the phase shift can be recorded simultaneously, offering further “probes” for detecting different sample properties.

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